The Escherichia coli remained the same color, so it was also urease negative Figure Escherichia coli is mainly found in animal feces and comprises their intestines as well US Food and Drug Administration.
The Proteus vulgaris exhibited growth above the inoculation line, turned black, and showed a red ring at the top of the solution, so it is motile, a phosphorus reducer, and an indole producer Figure The nine tests performed on the microbe were done according to the index of the virtual unknown software and the results of each test correlated to the results anticipated.
Catalase is tested for by adding hydrogen peroxide to the culture, and looking for the production of gas bubbles. Excerpt from Enterobacter Infections.
Another error could have been possibly occurred by mislabeling the plates according to species, Unknown lab report would produce invalid results. Several hypotheses arise during this experiment due to the many subjects being tested. The reagent does not mix with water and forms a thin layer above the broth.
Although this Unknown lab report went rather smoothly, there is always an opportunity for mprovement. The tests performed and what constitutes a positive and negative test are as follows.
However, if the medium does not change after the addition of the zinc, the unknown is positive for nitrate reduction, as the nitrite has just been further reduced, preventing its detection.
As shown, it was Figure 12 is Bacillus cereus for lactose. However, since there are numerous tests being performed, a more general hypothesis can be ascertained.
It is selective for salt tolerance and differential for maintol sugar fermentation. Red medium on the second addition indicates nitrate was not reduced and a negative test result.
Procedures were followed as stated in the index of the Virtual Unknown Software. Iodine was the dropped on the cultures to react with the starch and form a dark brown color.
The Escherichia coli exhibited growth, so it is Gram negative, and it turned red, so it ferments lactose Figure Opportunist pathogens are pathogens that do not cause much damage to healthy individuals; however, it can cause very severe damage to those with a weakened immune system.
Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures.
As shown, it was ring at the top that fades to yellow at the bottom, was cloudy orange throughout, had darker solution inside the tube than out, all the way through, and had no bubbles. As shown, it had a dark yellow and cloudy throughout, and had no bubbles.
For Part E, use a sterile swab to transfer the cells from Enterobacter aerogenes and Pseudomonas fluorescens to a disk. After concluding that the microbe was Gram negative rods, it was inoculated into a previously prepared test tube.
This process is commonly used in the biodegration industry. As shown, it was uniformly Figure 10 is Enterobacter aerogenes for lactose. As shown, it was red yellow, cloudy at the top, and had bubbles. Microbiology Unknown 1 was found in a nearby pond that was created by an earthquake.
In the Cytochrome Oxidase test, the Enterbacter aerogenes did not change color, so it is cytochromoe oxidase negative Figure The control also turned red, so the nitrate was not reduced by nitrate reductase, meaning it was also nitrate reductase negative Figure The hypothesis for all tests in both Lab 7 and Lab 8 is that the outcome of the tests will produce the desired results in order to differentiate various species of bacteria from one another and to reveal certain characteristics of metabolic and biochemical processes.
Materials and Methods Lab 7 For Part A of Lab 7, label Escherichia coli, Proteus vulgaris, the unknown, and Enterobacter aerogenes on a blue sucrosea green glucoseand a red lactose tube.Microbiology Unknown Lab Report. Samantha Moellenhoff.
Microbiology, Fall INTRODUCTION. With the constant debate in society about microorganisms, it is important to distinguish their identities. Report Sheet: Identification of Unknown Organic Compounds A.
Characterizing Known Compounds Carefully record your observations. Accurately describe each positive test and include factors such. Unknown Lab Report Margaret E Gibson July 20, Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests.
Unknown Microbiology Report Words | 4 Pages. The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity.
View Lab Report - Example Unknowns Lab Report from CHEM at Wesleyan University. Organic Chemistry Lab Final Project: Identification of A Solid Unknown Unknown M OH Abstract Bunsen. Example of a Microbiology Unknown Lab Report by Taylor Autry Introduction.
In this paper I will discuss the processes of how I came to find my two unknown bacteria.Download